Electrophysiological Effects of Voltage Sensitive Dye di-4-ANNEPS in Rabbit and Guinea Pig Isolated Heart
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Analysis of Biomedical Signals and Images |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Keywords | voltage sensitive dye; isolated heart |
| Description | Voltage-sensitive dye (VSD) di-4-ANEPPS is currently used in our laboratory for recording of monophasic action potentials (MAPs) in isolated hearts. In this study, its electrophysiological effects on rabbit and guinea pig myocardium were compared during staining with VSD and washout from it. Electrograms were recorded and heart rate changes and the types and frequency of arrhythmias were evaluated. Althought certain electrophysiological changes were present in the hearts of both species, these changes are mostly reversible, especially in rabbit hearts. Thus, rabbit myocardium can be considered a reliable model for electrophysiological studies, especially when generation and propagation of the impulse is studied. It may be concluded that rabbit isolated heart is more suitable model for electrophysiological studies in the isolated heart model than guinea pig heart. |
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