Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

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Authors

KOLÁŘOVÁ Jana FIALOVÁ Kateřina JANOUŠEK Oto NOVÁKOVÁ Marie PROVAZNÍK Ivo

Year of publication 2010
Type Article in Periodical
Magazine / Source Physiological Research
MU Faculty or unit

Faculty of Medicine

Citation
Web http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=P2OFFFD6GA56C3HOpNO&page=1&doc=1
Field Physiology
Keywords Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium
Description Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
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