Determination of Metallothionein in prostate tumor cell lines

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Authors	GUMULEC Jaromír MASAŘÍK Michal KUCHTÍČKOVÁ Šárka KUDLÁČKOVÁ Veronika JURAJDA Michal PAVLÍK Dušan ROVNÝ Arne HRABEC Roman KŘÍŽKOVÁ Soňa KIZEK René
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Medicine
Citation
Description	Prostate carcinoma (PCa) is the most common tumor and second leading cause of death due to cancer of men. Proteins such as metallothionein (MT) or alpha-methyl-CoA-racemase (AMACR) are highly overexpressed in prostate carcinoma. Althrough the role of these proteins in tumour tissue remains still unclear, it fulfills the requirements of higher specificity, which AMACR achieves at almost 100%. We have analyzed both proteins from prostate cancer cell lines LNCaP, PC-3 and 22RVL, compared to healthy prostate cell line PNT1A and then from PCa patients' serum compared to healthy serum controls. We've used immunohistochemical analysis, immunoprecipitation with magnetic nanoparticles, PAGE and dot blots. In all of these methods, we've found significant increase of expression in these samples.
Related projects:	Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu Nádorové markery, jejich stanovení a korelace s karcinomem prostaty