Mitochondrial Toxicity of Microcystin-LR on Cultured Cells: Application to the Analysis of Contaminated Water Samples

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	JASIONEK G. ZHDANOV A. DAVENPORT J. BLAHA L. PAPKOVSKY D.B.
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Environmental Science & Technology
MU Faculty or unit	Faculty of Science
Citation
Field	Environment influence on health
Keywords	microcystin; cyanobacteria; bioanalysis; mitochondria
Description	We analyzed the effects of MC-LR on primary hepatocytes, HepG2, and Jurkat T cells, and isolated rat liver mitochondria by measuring changes in 02 consumption by optical oxygen sensing technique. The respiratory response did not coincide with changes in viability, total cellular ATP, extracellular acidification, ROS formation, or protein phosphorylation, which were detectable at higher MC-LR doses. Such prominent effect on cellular respiration was therefore used for the detection of MC-LR in environmental samples.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)