Use of flow cytometry in research on apomictic plants
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Preslia |
| MU Faculty or unit | |
| Citation | |
| Field | Botany |
| Keywords | agamic complexes; cytotype; evolution; flow cytometry; gentic control of apomixis; genome size; hybridization; parthenogenesis; polyploidy; residual sexuality |
| Description | This paper reviews recent use of flow cytometry in studies on apomictic plant taxa. The most of apomictic angiosperms are polyploid, often differing in ploidy level from their sexual counterparts within the agamic complex. Flow cytometry is widely used for screening the ploidy levels of mature plants and their seed generated both in the field and in experiments. The diversity in modes of reproduction in apomicts is commonly reflected in the ploidy structure of their progeny in mixed-cytotype populations. Collectively, flow cytometry has been used to address the following research topics: (i) assessing the variation in ploidy levels and genome sizes in agamic complexes, (ii) detection and quantification of different reproductive modes in facultative apomicts, (iii) elucidation of processes in populations with coexisting sexual and apomictic biotypes, (iv) evolution of agamic complexes, and (v) genetic basis of apomixis. |
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