Physiological study of Antarctic green algal lichen photobionts: An optimized method of isolation and culturing technique
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Proceedings |
| Conference | Structure and Function of Antarctic Terrestrial Ecosystems. Book of Abstracts and Contributed Papers |
| MU Faculty or unit | |
| Citation | |
| Field | Botany |
| Keywords | isolation techniques; culturing; lichen symbiont |
| Description | Photobiont cells can be more or less successfully isolated from all lichens. Knowledge of lichen anatomy offers important information for isolation of photobionts. In our laboratory, we have been dealing with algal partner cultivation for some 6 years. Here we bring an overview of the method used with few optimizing points. For photobiont isolation from lichen thalli, we optimize the techniques of differential centrifugation combined with subsequent filtration of cell suspension. |
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