RP-HPLC with off-line MALDI MS detection of selected sterols

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Authors	HÉGROVÁ Blanka PEŠ Ondřej YEUNG Edward S. PREISLER Jan
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation	HÉGROVÁ, Blanka, Ondřej PEŠ, Edward S. YEUNG and Jan PREISLER. RP-HPLC with off-line MALDI MS detection of selected sterols. In 25th International Symposium on Microscale Bioseparation MSB 2010. 2010. ISBN 978-80-254-6631-5.
Description	Chromatograhic separation of sterols was optimalised for their fraction collection and subsequent MALDI MS determination using silver nanoparticles as matrix. The off-line HPLC-MALDI MS was found to be suitable for analysis of nonpolar compounds and can be utilized as alternative technique to gas chromatography.
Related projects:	Molecular basis of cell and tissue regulations Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine Multidetection platform for microcolumn separation of proteins and peptides