A Multidetection Platform for Microcolumn Separations

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	PREISLER Jan JUNGOVÁ Pavla PEŠ Ondřej KANICKÝ Viktor NAVRÁTILOVÁ Jarmila ŠMARDA Jan KRÁSENSKÝ Pavel VACULOVIČ Tomáš
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	An instrumental platform based on a universal off-line interface for deposition of capillary electrophoresis (CE) or liquid chromatography (LC) effluent on a suitable target will be described. The deposited fractions of analytes, such as peptides, proteins, metalloproteins or inorganic compounds may be analyzed in off-line mode using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Laser-induced fluorescence (LIF) detection may be used for visualization of analytes on target. Analytes may be subjected to reactions on target, such as digestion for protein identification. Additionally, inductively-coupled plasma mass spectrometry (ICP MS) detection mode, nature of which is complementary to soft MALDI MS, may be exploited.
Related projects:	Molecular basis of cell and tissue regulations Multidetection platform for microcolumn separation of proteins and peptides