A Multidetection Platform for Microcolumn Separations

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Authors

PREISLER Jan JUNGOVÁ Pavla PEŠ Ondřej KANICKÝ Viktor NAVRÁTILOVÁ Jarmila ŠMARDA Jan KRÁSENSKÝ Pavel VACULOVIČ Tomáš

Year of publication 2010
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description An instrumental platform based on a universal off-line interface for deposition of capillary electrophoresis (CE) or liquid chromatography (LC) effluent on a suitable target will be described. The deposited fractions of analytes, such as peptides, proteins, metalloproteins or inorganic compounds may be analyzed in off-line mode using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Laser-induced fluorescence (LIF) detection may be used for visualization of analytes on target. Analytes may be subjected to reactions on target, such as digestion for protein identification. Additionally, inductively-coupled plasma mass spectrometry (ICP MS) detection mode, nature of which is complementary to soft MALDI MS, may be exploited.
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