A model of in vitro drug metabolism testing by in-capillary enzymatic reaction utilizing capillary electrophoresis
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| Year of publication | 2010 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | High throughput screening methods are desired as a number of potential new drug compounds is large. Recombinant human cytochrome P450 (CYP) enzymes belong to favorable in vitro test system. As a model system, the metabolism pathway of dextromethorphan was chosen as an example of drug transformation under the action of cytochrome P450 2D6 (CYP 2D6). This enzyme belongs to important isoforms of hepatic CYP enzymes as it is involved in metabolism of about 25% of all commonly prescribed drugs. Capillary electrophoretic (CE) techniques offer direct automated assays. Moreover, CE enables to conduct in-line homogenous enzymatic reactions. The reaction and the separation can take place in capillary at the same time or consecutively. The aim of our study was to develop in-capillary CYP 2D6 enzymatic assay. The model assay was focused on characterization of initiation of enzymatic reaction to reach the maximal metabolite production. |
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