Sensing of human plasma fibrinogen on polished, chemically etched and carbon treated titanium surfaces by diffractive optical element based sensor.

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Authors

SILVENNOINEN Raimo VETTERL Vladimír HASOŇ Stanislav TUONONEN Hejkli SILVENNOINEN Martti MYLLER Kari CVRČEK Ladislav VANĚK Jiří PRACHÁR Patrik

Year of publication 2008
Type Article in Periodical
Magazine / Source Optics Express
MU Faculty or unit

Faculty of Medicine

Citation
Field Biophysics
Keywords PROTEIN ADSORPTION; PHASE TRANSIENTS; IMPLANT SURFACES; IN-VITRO; OXIDE; DIAGNOSTICS; ACTIVATION; ROUGHNESS; ADENOSINE; CERAMICS
Description Adsorption of human plasma fibrinogen (HPF) on 6 differently treated titanium samples (polished, polished and etched, and 4 titanium carbide coatings samples produced by using plasma-enhanced chemical vapour deposition (PECVD) method) is investigated by using diffractive optical element (DOE) sensor. Permittivity (susceptibility) change and fluctuation in optical roughness (R-opt) of treated titanium surface in the presence of background electrolyte without and with HPF molecules are sensed by using DOE sensor and optical ellipsometry. Correlation between transmitted light and thickness of molecule layer was found. The findings allow to sense temporal organization and severity of adsorption of nano-scale HPF molecules on polished, on polished and etched, and on titanium carbide surface.
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