Simultaneous HPLC determination of tolbutamide, phenacetin and their metabolites as markers of cytochromes 1A2 and 2C6/11 in rat liver perfusate

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Authors	JUŘICA Jan KONEČNÝ Jiří ZAHRADNÍKOVÁ Lucia TOMANDL Josef
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Journal of Pharmaceutical and Biomedical Analysis
MU Faculty or unit	Faculty of Medicine
Citation
Web	http://dx.doi.org/10.1016/j.jpba.2010.01.028
Field	Biochemistry
Keywords	CYP2C6; CYP1A2; metabolic activity; marker
Description	The new original method for assessment the metabolic activity of both rat CYP1A2 and CYP2C6/11 is presented in the paper. The method of simultaneous determination of phenacetin, tolbutamide and their metabolites is rapid simple, repeatable and precious and sensitive enough for pharmacokinetic studies.
Related projects:	The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions. Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie Miniaturized on-line drug metabolism system based on capillary electrophoresis