Molekulární detekce lokusu makrolidové rezistence u klinických izolátů syfilis v České republice.
| Title in English | Molecular detection of locus of macrolide resistance in syphilis clinical isolates from Czech Republic. |
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| Authors | |
| Year of publication | 2009 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Primary screening of clinical specimen included nested PCR detection of two T. pallidum specific loci (tmpC, polA). In PCR positive samples, 23S rDNA locus comprising position 2058 was amplified. The amplicon was subjected to Sanger sequencing using original amplification primers with the aim to detect mutations in corresponding part of the 23S rRNA gene. The set of 28 clinical isolates with detectable genetic material were collected from 22 patients in the Czech Republic in the time period 2005-2008. 14 patients (63.6%) were infected with macrolide sensitive strain of T. pallidum. Four patients (18.2%) were diagnosed with a strain bearing the A2058G transition and four patients (18.2%) with a strain bearing the A2059G mutation (spiramycin treatment failure was reported in one of these patients). Screening of clinical samples revealed a novel mutation at the position 2059 of the T. pallidum 23S rRNA gene. This mutation causes macrolide resistance in several other bacteria. Presented results show that macrolide resistant isolates of T. pallidum bearing A2058G and A2059G mutations are relatively abundant in the Czech Republic. High probability of macrolide treatment failure emphasizes the need of the follow-up of syphilis patients. |
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