Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Diagnostic Microbiology and Infectious Disease |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6T60-4XNDB8V-6&_origin=SDEMFRHTML&_version=1&md5=01279d3a76e20c072c2a3c4a3d798f31 |
| Field | Genetics and molecular biology |
| Keywords | Staphylococcus aureus; Pemphigus neonatorum; Exfoliative toxin A; Prophages; Multiplex PCR |
| Description | The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control. 2010 Elsevier Inc. All rights reserved. |
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