In Vitro Activation of Cytotoxic T Lymphocytes By Muc1 Pulsed Dendritic Cells
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | The Open Cancer Immunology Journal |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | multiple myeloma; dendritic cell; Muc1; immunotherapy |
| Description | We studied antigen-specific and HLA-A2-restricted cytotoxic activity against MUC1-positive ARH77 myeloma cell line in vitro. A HLA-A2 specific MUC1-derived nonapetide (139TSAPDTRPA147) was used as a tumor-associated antigen. Myeloma-specific cytotoxic activity of MUC1-reactive CTL was established by repeated stimulation of CTL via dendritic cells loaded with MUC1-derived nonapeptide. We were able to demonstrate that MUC1-reactive T cells can be identified and expanded using a relatively simple in vitro techniques. Antigen-specific stimulation via dendritic cells, immunomagnetic sorting and rapid expansion of CTL without loosing of their specific immune activity can be used clinically even in poorly immunogenic diseases such as MM. |
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