Random mutagenesis as a tool for fine-tuning of affinity and specificity of PA-IIL lectin from Pseudomonas aeruginosa
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| Year of publication | 2009 |
| Type | Conference abstract |
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| Description | Directed evolution is a powerful tool widely used for elucidating the relationship between protein sequence, structure and function and also for producing proteins with altered or improved properties. The most useful approach in directed evolution is a random mutagenesis, which includes a lot of methods used to create diverse mutant libraries. LecB is the target gene we have modified via random mutagenesis, the gene coding PA-IIL lectin. This Pseudomonas aeruginosa soluble protein has been well-characterized. The random mutagenesis could help us to create a protein with interestingly modified specificity and affinity. After several trials we obtained an efficient method which introduces non-silent random mutations into the gene of our interest in sufficient rate. The mutant lectins with improved properties can be used in biotechnology and for bioanalytical purposes. |
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