Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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Authors	DYČKA Filip LAŠTOVIČKOVÁ Markéta ČERNÝ Martin BRZOBOHATÝ Břetislav BOBÁLOVÁ Janette
Year of publication	2009
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation	DYČKA, Filip; Markéta LAŠTOVIČKOVÁ; Martin ČERNÝ; Břetislav BRZOBOHATÝ and Janette BOBÁLOVÁ. Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In 3rd Central and Eastern European Proteomics Conference. Book of Abstracts. 2009. ISBN 978-963-9319-99-8.
Description	In the present study, two methods for enrichment of tryptic phosphopeptides were described: IMAC and titanium dioxide chromatography. After enrichment procedure the phosphopeptides were analyzed by MALDI-TOF MS. Alpha-casein was used as model phosphoprotein. The methods were applied to protein mixture of Arabidopsis thaliana.
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