Separation of PCR-ready DNA from dairy products using magnetic hydrophilic microspheres and poly(ethylene glykol)-NaCl water solutions

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Authors

RITTICH Bohuslav ŠPANOVÁ Alena ŠÁLEK Petr NĚMCOVÁ Petra TRACHTOVÁ Štěpánka

Year of publication 2009
Type Article in Periodical
Magazine / Source Journal of Magnetism and Magnetic Materials
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Magnetic microsphere; P(HEMA-co-GMA); DNA isolation; dairy product; polymerase chain reaction (PCR)
Description Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co- glycidyl dimethylmethacrylate) (P(HEMA-co-GMA)) and magnetic glass microspheres were used for the isolation of bacterial DNA. The most extensive DNA recovery achieved at PEG 6000 concentrations of 12% or 16 % and 2 M NaCl. The method proposed was used for bacterial DNA isolation from different dairy products containing Bifidobacterium and Lactobacillus cells. The presence of target DNA and quality of isolated DNA was checked by PCR amplification with specific primers.
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