Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Proceedings |
| Conference | Moderní elektrochemické metody |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | 7-deaza purines; electrochemical detection; carbon electrode; repetitive elements |
| Description | 7 deaza purines (particularly 7 deaza guanine) incorporated into the DNA sequence during in vitro extension and/or PCR enable the simple electrochemical detection of the PCR products at the carbon eletrode. Based on the pilot experiments, the utilization of the method in a fast and simple analysis of G rich repetitive sequences in eukaryotic genomes is supposed. |
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