MALDI MS profiling of bacteria

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Authors	ŠEDO Ondrej VORÁČ Aleš TESHIM Andrea NEMEC Alexandr TVRZOVÁ Ludmila SEDLÁČEK Ivo ZDRÁHAL Zbyněk
Year of publication	2009
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	MALDI MS of intact bacterial cells or their lysates appears to be a highly promising tool for bacterial identification. During the past decade, numerous laboratories demonstrated the ability of this technique to rapidly resolve bacterial samples, concerning mostly a model set of organisms characterised previously or in parallel by other taxonomical approaches. Almost every laboratory developed its own sample preparation protocol working in a satisfactory way on a particular system of interest. Many factors were found to influence the signal quality, sensitivity, reproducibility and other method characteristics resulting in notable changes in MALDI MS profiling discriminatory power.
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