The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses

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Authors

KILIAN Joachim WHITEHEAD Dion HORÁK Jakub WANKE Dierk WEINL Stefan BATISTIC Oliver D´ANGELO Cecilia BORNBERG-BAUER Erich KUDLA Jörg HARTER Klaus

Year of publication 2007
Type Article in Periodical
Magazine / Source The Plant Journal
MU Faculty or unit

Faculty of Science

Citation
Web http://www3.interscience.wiley.com/cgi-bin/fulltext/118488525/HTMLSTART
Field Genetics and molecular biology
Keywords AtGenExpress; bioinformatics; gene expression; microarray; abiotic stress
Description The tolerance responses of plants to many abiotic stresses are conjectured to be controlled by complex gene networks. In the frame of the AtGenExpress project a comprehensive Arabidopsis thaliana genome transcript expression study was performed using the Affymetrix ATH1 microarray in order to understand these regulatory networks in detail. In contrast to earlier studies, we subjected, side-by-side and in a high-resolution kinetic series, Arabidopsis plants, of identical genotype grown under identical conditions, to different environmental stresses comprising heat, cold, drought, salt, high osmolarity, UV-B light and wounding. Here we describe the technical performance of the experiments. We also present a general overview of environmental abiotic stress-induced gene expression patterns and the results of a model bioinformatics analysis of gene expression in response to UV-B light, drought and cold stress.
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