Rozlišení nádorových klonálních a fyziologických polyklonálních plazmatických buněk pomocí flowcytometrie

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Title in English Flow cytometric discrimination between neoplastic clonal and physiological polyclonal plasma cells
Authors

KOVÁŘOVÁ Lucie BUREŠOVÁ Ivana SUSKÁ Renata MICHÁLEK Jaroslav PENKA Miroslav HÁJEK Roman

Year of publication 2008
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.linkos.cz/vzdelavani/Supp1_08/22.pdf
Field Oncology and hematology
Keywords plasma cells; multiple myeloma; monoclonal gammopathy of undetermined significance; flow cytometry
Description Multicolor flow cytometry is a sensitive tool for the analysis of plasma cell population. It can differentiate between normal and malignant plasma cells and enables their quantification. We have used flow cytometry for enumeration and immunophenotyping of plasma cells from 25 patients with monoclonal gammopathy of unknown significance and 78 those with multiple myeloma aiming to identify and characterize markers that distinguish between the two conditions. The expression of surface antigens CD38, CD138, CD45, CD56 and CD19 was analysed on bone marrow plasma cells. As compared to MM patients, MGUS patients had higher proportion of residual CD19+ plasma cells (p<0,001) and lower counts of pathologic CD56+ plasma cells (p=0,004) in the bone marrow. The percentage of CD19+ plasma cells appears to be an unequivocal marker that distinguishes monoclonal gammopathy of unknown significance from all stages of multiple myeloma. Immunophenotyping of plasma cell can thus contribute to the differential diagnosis of both diseases. It has also been suggested that flow cytometric analysis will be useful for prognostic evaluation and for the study of minimal residual disease.
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