Rozlišení nádorových klonálních a fyziologických polyklonálních plazmatických buněk pomocí flowcytometrie
| Title in English | Flow cytometric discrimination between neoplastic clonal and physiological polyclonal plasma cells |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Klinická onkologie |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.linkos.cz/vzdelavani/Supp1_08/22.pdf |
| Field | Oncology and hematology |
| Keywords | plasma cells; multiple myeloma; monoclonal gammopathy of undetermined significance; flow cytometry |
| Description | Multicolor flow cytometry is a sensitive tool for the analysis of plasma cell population. It can differentiate between normal and malignant plasma cells and enables their quantification. We have used flow cytometry for enumeration and immunophenotyping of plasma cells from 25 patients with monoclonal gammopathy of unknown significance and 78 those with multiple myeloma aiming to identify and characterize markers that distinguish between the two conditions. The expression of surface antigens CD38, CD138, CD45, CD56 and CD19 was analysed on bone marrow plasma cells. As compared to MM patients, MGUS patients had higher proportion of residual CD19+ plasma cells (p<0,001) and lower counts of pathologic CD56+ plasma cells (p=0,004) in the bone marrow. The percentage of CD19+ plasma cells appears to be an unequivocal marker that distinguishes monoclonal gammopathy of unknown significance from all stages of multiple myeloma. Immunophenotyping of plasma cell can thus contribute to the differential diagnosis of both diseases. It has also been suggested that flow cytometric analysis will be useful for prognostic evaluation and for the study of minimal residual disease. |
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