A label free piezoelectric immunosensor for rapid assay of Escherichia coli
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Immunoassay and Immunochemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Piezoelectric biosensor; Quartz crystal microbalance; Immunoassay; Immunosensor; Escherichia coli |
| Description | piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 106-109 CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 108 CFU/mL. |
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