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Title in English Isolation of Plasma Cells
Authors

ČUMOVÁ Jana BUREŠOVÁ Ivana KOVÁŘOVÁ Lucie KYJOVSKÁ Drahomíra VIDLÁKOVÁ Petra SUSKÁ Renata PERUTKA Tomáš MORAVCOVÁ Jana RYCOVÁ Marcela PENKA Miroslav HÁJEK Roman

Year of publication 2008
Type Article in Periodical
Magazine / Source Klinická onkologie Supplement 2008
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Keywords Multiple myeloma;Separation;MACS;FACS;CD138
Description We have used magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) for the separation of plasma cells (PC) from bone marrow aspirates from patients with multiple myeloma (MM) using human antibodies CD138 MACS Micro- Beads or CD138-PE antibodies that recognize the plasma cell surface antigen CD138 (syndecan-1). Isolated PC can be used for molecular biology studies of MM such as PCR analysis, comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), protein analysis, analysis of cell surface markers, and/or intracellular antigens.
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