Significant overexpression of Hsp110 gene during colorectal cancer progression.
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Oncology Reports |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.spandidos-publications.com/or/ |
| Field | Oncology and hematology |
| Keywords | colorectal cancer; staging; lymph nodes; progression; gene expression profiles; Hsp110 |
| Description | Colorectal cancer (CRC) is one of the most frequent malignant disease in the world. Metastatic spread of the cancer to the lymph nodes is crutial factor for progression and therapeutic management of the disease. We suppose that analysis of gene expression profiles of CRC patiens by low-density cancer-focused oligonucleotide microarrays enable us to identify new predictive markers of the extend of disease and can lead to a better understanding of CRC progression. Relative expression levels of 440 genes known to be involved in cancer biology were obtained by low-density oligonucleotide microarrays from 20 tumor samples. Statistical analysis of gene expression data identified 3 genes (HSP110, HYOU1, TCTP) significantly up-regulated in primary tumors of patients who developed lymph nodes metastasis. We have shown, for the first time, that up-regulation HSP110 and HYOU1 expression is associated with lymph nodes involvement in CRC. We validated the differences in HSP110 expression in an independent group of 30 patients of all clinical stages by Real-Time PCR method. We identified significant up-regulation of HSP110 expression in colorectal tumors compared to adjacent non-tumoral tissue (p<0,0003). For the first time, we observed significant differences of HSP110 gene expression between metastatic and localized disease (p=0,031) and negative trend of HSP110 gene expression and overall survival of CRC patients. We suggest that HSP110 gene is promising molecular predictor in CRC. |