Studium strukturálních změn membrán buněk HL-60 metodou freeze-etching
| Title in English | Study of structural changes in membranes of HL-60 cells using freeze-etching method |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Sborník příspěvků XXXI. Dny lékařské biofyziky |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | plasma membrane; nuclear envelope; HL-60 cells; ultrasound; freeze-etching |
| Description | Freeze-etching method was used to observe structure of membrane areas HL-60 cells. The attention was concentrated on the plasma membrane and nuclear envelope. Replicas of cells affected by ultrasound and handling conditions were observed, distribution of proteins in the area of plasma membrane and shape of the membrane and the distribution of nuclear pores in the nuclear envelope were studied. Cell respond to the mechanical stress affected by ultrasound is similar as a result of handling with cell culture i.e. local corrugation membrane surface. |
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