Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

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Authors	TRACHTOVÁ Štěpánka ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2008
Type	Article in Proceedings
Conference	International Probiotic Conference. Pediatria 2, 2008.
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	ethidium monoazide; dead and live bacterial cells
Description	The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.
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