Optimalizace reversibilní adsorpce vysokomolekulární DNA na magnetické nosiče

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Title in English Optimization of reversible adsorption of high-molecular DNA on magnetic microspheres
Authors

ŠÁLEK Petr TRACHTOVÁ Štěpánka ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2008
Type Article in Proceedings
Conference XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords DNA; reversible adsorption; magnetic microspheres; P(HEMA-co-GMA); carboxyl coated
Description Genomic DNA were isolated from crude cell lysates of Lactobacillus paracasei subsp. paracasei CCDM 211/06 cells with help of reversible imobilisation on hard carriers. Magnetic nonporous micropheres poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA)covered by carboxyl groups were used for DNA isolation. DNA adsorption on carriers occured in the presence of high NaCl and PEG 6000 concentrations. Quality and amount of isolated DNA were estimated using agarose gel electrophoresis, spectrophotometry and amplification in PCR.
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