Využití PCR při rozlišení živých a mrtvých bakteriálních buněk

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Title in English	Differentiation of live and dead bacterial cells by PCR
Authors	TRACHTOVÁ Štěpánka ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2008
Type	Article in Proceedings
Conference	XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation	TRACHTOVÁ, Štěpánka, Alena ŠPANOVÁ and Bohuslav RITTICH. Využití PCR při rozlišení živých a mrtvých bakteriálních buněk (Differentiation of live and dead bacterial cells by PCR). In XII. Setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita, Brno - Přírodovědecká fakulta, 2008, p. 106. ISBN 978-80-210-4526-2.
Field	Genetics and molecular biology
Keywords	PCR; EMA; live and dead cells;
Description	Lactobacillus paracasei ssp.paracasei CCDM211/06 were used for confirmation of the method. Different methods were used for preparation of dead cells (control). The time of incubation of cell suspension with EMA as the time of irradiation of the sample with halogen lamp were tested. PCR was carried out using genus specific primers (Dubernet a spol., 2002).
Related projects:	Molecular basis of cell and tissue regulations Aplication of molecular biology methods for the effective use of genetic sources of LAB and the characterisation of functional foods quality