Monodisperse thermosensitive poly(N-iso-propylacrylamide) microspheres for isolation of genomic DNA

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Authors

RITTICH Bohuslav ŠPANOVÁ Alena MACKOVÁ Hana HORÁK Daniel

Year of publication 2008
Type Article in Proceedings
Conference 10 th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers & 10 th International Symposium on Advances in Extraction Techniques 2008
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords P(NIPAAm-co-GMA-co-MBAAm); thermosensitive microspheres; DNA adsorption; temperature influence
Description The monodisperse thermosensitive P(NIPAAm-co-GMA-co-MBAAm) microspheres (with and without carboxyl groups) were tested for sorption of DNA macromolecules. On the particles there was adsorbed condensed DNA from chicken erythrocytes (model) and bacterial DNA in the presence of high concentrations of NaCl and PEG 6,000 at 10 and 40 C; the adsorption of DNA was low at 10 C. The adsorbed DNA was then released from the microspheres by TE buffer (10 mM Tris.HCl; pH 7.8; 1 mM EDTA, pH 8.0) at laboratory temperature. DNA adsorption on the particles was detected using agarose gel electrophoresis in TBE buffer (45 mM boric acid, 45 mM Tris-base, 1 mM EDTA, pH 8.0) and confirmed by UV spectrophotometry.
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