Monodisperse thermosensitive poly(N-iso-propylacrylamide) microspheres for isolation of genomic DNA
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | 10 th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers & 10 th International Symposium on Advances in Extraction Techniques 2008 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | P(NIPAAm-co-GMA-co-MBAAm); thermosensitive microspheres; DNA adsorption; temperature influence |
| Description | The monodisperse thermosensitive P(NIPAAm-co-GMA-co-MBAAm) microspheres (with and without carboxyl groups) were tested for sorption of DNA macromolecules. On the particles there was adsorbed condensed DNA from chicken erythrocytes (model) and bacterial DNA in the presence of high concentrations of NaCl and PEG 6,000 at 10 and 40 C; the adsorption of DNA was low at 10 C. The adsorbed DNA was then released from the microspheres by TE buffer (10 mM Tris.HCl; pH 7.8; 1 mM EDTA, pH 8.0) at laboratory temperature. DNA adsorption on the particles was detected using agarose gel electrophoresis in TBE buffer (45 mM boric acid, 45 mM Tris-base, 1 mM EDTA, pH 8.0) and confirmed by UV spectrophotometry. |
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