Processing of a Large Number of FISH and Immunofluorescence Stained Cells on Confocal Spinning Disk Systems

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Authors	MATULA Pavel MAŠKA Martin DANĚK Ondřej MATULA Petr KOZUBEK Michal
Year of publication	2008
Type	Conference abstract
MU Faculty or unit	Faculty of Informatics
Citation
Description	We have developed a system for acquisition and analysis of a large number of 3D images of cells stained by fluorescent dyes. The system is a successor of a previously developed system for high-resolution cytometry of FISH-stained cells [1]. The new system, called Acquiarium [2], is capable of fast 3D acquisition of confocal images using microscopes with spinning disks and provides means for convenient analysis of many 3D image data in one collection. In comparison to the previous system, it was significantly improved; [1] M. Kozubek, S. Kozubek, et al., Combined confocal and wide-field high-resolution cytometry of FISH-stained cells, Cytometry, 2001, 45, 1-12 [2] Acquiarium 1.0: http://cbia.fi.muni.cz/projects/acquiarium-version-1.0.html
Related projects:	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images