aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

ŠEBKOVÁ Alena KARASOVÁ Daniela CRHÁNOVÁ Magdaléna BUDINSKÁ Eva RYCHLÍK Ivan

Year of publication 2008
Type Article in Periodical
Magazine / Source JOURNAL OF BACTERIOLOGY
MU Faculty or unit

Faculty of Science

Citation
Web http://jb.asm.org/cgi/content/abstract/190/9/3155
Doi http://dx.doi.org/10.1128/JB.00053-08
Field Microbiology, virology
Keywords aro mutant; Salmonella enterica; microarray; gene expression; plasmid
Description In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info