Determination of Asymmetric Dimethylarginine in Human Plasma - Comparison HPLC Methods with Fluorescent or Coulometric Detection with ELISA Method
| Title in English | DETERMINATION OF ASYMMETRIC DIMETHYLARGININE IN HUMAN PLASMA - COMPARISON HPLC METHODS WITH FLUORESCENT OR COULOMETRIC DETECTION WITH ELISA METHOD |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Sborník příspěvků |
| MU Faculty or unit | |
| Citation | |
| Web | XII. Setkání biochemiků a molekulárních biologů |
| Field | Biochemistry |
| Keywords | ADMA; HPLC; ELISA |
| Description | Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. The increase of concentration leads to increased blood pressure and reduced blood flow. Elevated plasma ADMA is an independent risk factor for endotelial dysfunction and has been associated with e.g. hypertension, diabetes mellitus, hypercholesterolemia, renal failure and atherosclerosis. In our correlation study we used EDTA-plasma samples from patients 24h after acute myocardial infarction and ADMA concentrations, obtained by HPLC with fluorescent or coulometric detection and ELISA method, were compared and among methods was poor correlation. Detection limits of HPLC methods was approximately 5times lower than in ELISA method and 50times lower than in plasma of healthy people. |
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