Determination of Asymmetric Dimethylarginine in Human Plasma - Comparison HPLC Methods with Fluorescent or Coulometric Detection with ELISA Method

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Title in English DETERMINATION OF ASYMMETRIC DIMETHYLARGININE IN HUMAN PLASMA - COMPARISON HPLC METHODS WITH FLUORESCENT OR COULOMETRIC DETECTION WITH ELISA METHOD
Authors

KLEPÁRNÍK Martin TOMANDL Josef

Year of publication 2008
Type Article in Proceedings
Conference Sborník příspěvků
MU Faculty or unit

Faculty of Medicine

Citation
Web XII. Setkání biochemiků a molekulárních biologů
Field Biochemistry
Keywords ADMA; HPLC; ELISA
Description Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. The increase of concentration leads to increased blood pressure and reduced blood flow. Elevated plasma ADMA is an independent risk factor for endotelial dysfunction and has been associated with e.g. hypertension, diabetes mellitus, hypercholesterolemia, renal failure and atherosclerosis. In our correlation study we used EDTA-plasma samples from patients 24h after acute myocardial infarction and ADMA concentrations, obtained by HPLC with fluorescent or coulometric detection and ELISA method, were compared and among methods was poor correlation. Detection limits of HPLC methods was approximately 5times lower than in ELISA method and 50times lower than in plasma of healthy people.
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