Determination of isoflavones using liquid chromatography with electrochemical detection

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Authors

MIKELOVÁ Radka HODEK Petr HANUŠTIAK Pavel ADAM Vojtěch KŘÍŽKOVÁ Soňa HAVEL Ladislav STIBOROVÁ Marie HORNA Aleš BEKLOVÁ Miroslava TRNKOVÁ Libuše KIZEK René

Year of publication 2007
Type Article in Periodical
Magazine / Source Acta Chimica Slovenica
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords Isoflavonoids; injection flow analysis; HPLC
Description Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 dg. C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.
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