Determination of isoflavones using liquid chromatography with electrochemical detection

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Authors	MIKELOVÁ Radka HODEK Petr HANUŠTIAK Pavel ADAM Vojtěch KŘÍŽKOVÁ Soňa HAVEL Ladislav STIBOROVÁ Marie HORNA Aleš BEKLOVÁ Miroslava TRNKOVÁ Libuše KIZEK René
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Acta Chimica Slovenica
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	Isoflavonoids; injection flow analysis; HPLC
Description	Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 dg. C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine