Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Medicine. Official publication website can be found on muni.cz.
Authors

GARAJOVÁ Ingrid FABIAN Pavel NENUTIL Rudolf KOCÁKOVÁ Ilona GRELL Peter HANZELKOVÁ Zina VYZULA Rostislav SVOBODA Marek

Year of publication 2007
Type Article in Proceedings
Conference Molecular Targets in Cancer Therapy:Mechanism and Therapeutic Reversal of Immune Suppression in Cancer
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Keywords FOXP-3 protein;T-regulatory lymphocytes;colorectal carcinoma
Description Tumor is a complex tissue composed of cancer cells and stromal cells (e.g. endothelial cells, fibroblasts, dendritic and NK cells, macrophages and lymphocytes). Tumor-infiltrating lymphocytes (TIL) are found in a variety of solid cancers and they are a possible prognostic factor as it is thought that TILs execute a host immune response against cancer cells. In colorectal carcinoma (CRC), TILS are particularly numerous in cases associated with microsatellite instability and have more favorable clinical outcome. Generally, cytotoxic T-cells (CD8+) are prognostically favorable, whereas recent discovered subgroup of TILs, regulatory T-cells (T-reg, CD4+CD25+FOXP3+) are not. They inhibit antitumor activity of CD8+ and CD4+ T-cells. The aim of our study was to investigate if the TIL of CRC include T-reg lymphocytes, which was not proved so far. More recent studies have shown that T-reg lymphocytes are unically characterized by expression of transcription factor FOXP3. Therefore we used immunohistochemical staining to detect lymphocytes co-expressing CD4+ and FOXP3+ in 9 cases of CRC primary tumors. All cases of CRC were left-side localized, respecting a different biological behaviour of left/right-side localized CRCs. We used formalin-fixed and paraffin-embedded sections and commercially available monoclonal antibodies. Our preliminary results show that TIL in CRC include in cancer stroma a subset of CD4+ FOXP3+ lymphocytes. Now, we are interested if T-reg lymphocytes can be used as a prognostic marker for CRC and we analyze a group of 40 patients with CRC in I-IV clinical stage. We are also interested if there is a connection between occurrence of T-reg and other stromal cells, especially cytotoxic T-lymphocytes and NK cells. This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.:NR/9076-4.

You are running an old browser version. We recommend updating your browser to its latest version.

More info