Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	RYVOLOVÁ Markéta PEŠ Ondřej FOHLEROVÁ Radka HEJÁTKO Jan PREISLER Jan
Year of publication	2007
Type	Article in Proceedings
Conference	CECE 2007 4th international interdisciplinary meeting on bioanalysis
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	capillary electrophoresis; GFP
Description	In this work, using laser diode (405 nm) as a prospective excitation source for monitoring of phosphorylation process of cytosolic histidine-containing phosphotransfer proteins (designated as AHP1-6 in Arabidopsis) has been suggested. To our knowledge, this laser emitting at 405 nm has not been applied for CE-LIF of GFP and/or GFP-tagged proteins yet.
Related projects:	Molecular basis of cell and tissue regulations Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine