Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins

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Authors

RYVOLOVÁ Markéta PEŠ Ondřej FOHLEROVÁ Radka HEJÁTKO Jan PREISLER Jan

Year of publication 2007
Type Article in Proceedings
Conference CECE 2007 4th international interdisciplinary meeting on bioanalysis
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords capillary electrophoresis; GFP
Description In this work, using laser diode (405 nm) as a prospective excitation source for monitoring of phosphorylation process of cytosolic histidine-containing phosphotransfer proteins (designated as AHP1-6 in Arabidopsis) has been suggested. To our knowledge, this laser emitting at 405 nm has not been applied for CE-LIF of GFP and/or GFP-tagged proteins yet.
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