Isolation of PCR-ready DNA from real samples containing PCR inhibitors

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Authors

ŠPANOVÁ Alena RITTICH Bohuslav HORÁK Daniel RYBNIKÁŘ Alois

Year of publication 2007
Type Article in Proceedings
Conference Biopowders; Advances in the Powder Technology
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords fungi; Trichophyton; nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA)
Description Hydrophilic magnetic particles coated by carboxyl groups were used for PCR-ready DNA isolation. DNA was adsorbed on magnetic particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations, washed and eluted into TE buffer (10 mM Tris-HCl, pH 7.8; 1 mM EDTA; pH 8.0). The eluted DNA was used directly for PCR amplification and identification of Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products) and lyophilisates, Trichophyton cells in mixed cultures and bacterial cells in sediments from Lake Baikal. It was also used in GMO testing in foods with success.
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