Izolace DNA z kultur bakterií pomocí zjednodušeného postupu (vysolování chloridem sodným)

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Title in English	DNA isolation from bacterial cultures using a simplified procedure (salting-out by sodium chloride)
Authors	NĚMCOVÁ Eva KŘÍŽOVÁ Jana RITTICH Bohuslav ŠPANOVÁ Alena
Year of publication	2007
Type	Article in Proceedings
Conference	XI. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Bifidobacterium; ARDRA; identification
Description	The aim of the work was to study the influence of different NaCl concentrations on the amount of DNA isolated from crude cell lysates of genus Lactobacillus and Bifidobacterium. The quality of isolated DNA was proved using genus specific PCR. The salting out procedure is quick methods of DNA isolation without use of toxic substances. Next, the influence of different procedures of DNA isolation ((phenol extraction, salting out using 6 M NaCl, purification using magnetic carriers P(HEMA-co-EDMA) and silica)) on RAPD and rep-PCR profiles was studied.
Related projects:	Molecular basis of cell and tissue regulations Molecular-genetic methods for identification of probiotic bakteria with good technologic, hygienic, and health properties