Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system

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Authors	RITTICH Bohuslav ŠPANOVÁ Alena NĚMCOVÁ Petra HORÁK Daniel
Year of publication	2007
Type	Article in Proceedings
Conference	Ninth International Symposium on Advances in Extraction Technologies
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	DNA; isolation; magnetic microspheres; P(HEMA-co-EDMA); aqueous two-phase system; PEG 6000; NaCl
Description	The aim of work was the isolation of PCR-ready DNA from dairy products by magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethylmethacrylate) (P(HEMA-co-EDMA)) microspheres in the presence of an aqueous two-phase system (composed of PEG 6000 and NaCl). DNA was apparently adsorbed on magnetic particles surface from two-phase aqueous (PEG 6000/NaCl) system. The sensitivity of PCR of the DNA isolation method tested was comparable with the phenol extraction method.
Related projects:	Molecular basis of cell and tissue regulations Magnetic stimuli-responsive hydrophilic polymer microspheres for biomedicine/bioengineering Aplication of molecular biology methods for the effective use of genetic sources of LAB and the characterisation of functional foods quality