Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

PETRLOVÁ Jitka MASAŘÍK Michal POTĚŠIL David ADAM Vojtěch TRNKOVÁ Libuše KIZEK René

Year of publication 2007
Type Article in Periodical
Magazine / Source Electroanalysis
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1002/elan.200603837
Field Electrochemistry
Keywords streptavidin ; avidin ; modified electrode ; electrochemistry ; protein ; carbon paste electrode
Description We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS).
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info