Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry

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Authors	PETRLOVÁ Jitka MASAŘÍK Michal POTĚŠIL David ADAM Vojtěch TRNKOVÁ Libuše KIZEK René
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Electroanalysis
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1002/elan.200603837
Field	Electrochemistry
Keywords	streptavidin ; avidin ; modified electrode ; electrochemistry ; protein ; carbon paste electrode
Description	We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS).
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine