Hydrophilic magnetic particles for molecular diagnostics

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Authors

ŠPANOVÁ Alena RITTICH Bohuslav HORÁK Daniel RYBNIKÁŘ Alois

Year of publication 2006
Type Article in Proceedings
Conference 6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers
MU Faculty or unit

Faculty of Science

Citation
Field Biotechnology
Keywords Magnetic carriers; DNA; Dairy products; PCR
Description Magnetic nonporous P(HEMA-co-EDMA) particles were prepared by dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) in the presence of maghemite nanoparticles. Magnetic nonporous P(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA) in the presence of colloidal oleic acid-coated magnetite (Fe3O4) particles. Subsequently, the hydroxy groups of the particles were oxidized with 2 wt.% aqueous solution of potassium permanganate under acidic conditions (2 M sulphuric acid). Prepared particles were used for isolation of bacterial DNA from real samples. DNA was adsorbed on particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations. DNA was eluted using TE buffer and used directly for PCR amplification and identification Lactobacillus and Bifidobacterium cells in dairy products, Trichophyton cells in lyophilisates, bacterial cells in sediments from Lake Baikal.
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