In situ hybridization for mRNA detection in Arabidopsis tissue sections.

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Authors	HEJÁTKO Jan BREWER P. HEISLER M. FRIML Jiří BENKOVÁ Eva
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Nature Protocols
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	in situ mRNA localization; tissue sections; plants
Description	Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days.
Related projects:	Molecular basis of cell and tissue regulations Regulation of morphogenesis of plant cells and organs