Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria

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Authors	RITTICH Bohuslav KŘÍŽOVÁ Jana ŠPANOVÁ Alena NĚMCOVÁ Petra HORÁK Daniel
Year of publication	2006
Type	Article in Proceedings
Conference	Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Magnetic particles; HEMA-co-GMA; Bifidobacterium; RAPD; rep-PCR; reproducibility
Description	DNA was isolated from Bifidobacterium cells using phenol extraction metod, salting out using 6 M NaCl, and adsorption on magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (HEMA-co-GMA) particles containing carboxyl groups. It was shown that DNA eluted from magnetic particles into TE buffer can be succesfully used in molecular fingerprinting procedures. The reproducibility of tested methods were comparable with phenol extraction method.
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