Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria

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Authors

RITTICH Bohuslav KŘÍŽOVÁ Jana ŠPANOVÁ Alena NĚMCOVÁ Petra HORÁK Daniel

Year of publication 2006
Type Article in Proceedings
Conference Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Magnetic particles; HEMA-co-GMA; Bifidobacterium; RAPD; rep-PCR; reproducibility
Description DNA was isolated from Bifidobacterium cells using phenol extraction metod, salting out using 6 M NaCl, and adsorption on magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (HEMA-co-GMA) particles containing carboxyl groups. It was shown that DNA eluted from magnetic particles into TE buffer can be succesfully used in molecular fingerprinting procedures. The reproducibility of tested methods were comparable with phenol extraction method.
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