Použití magnetických mikročástic pro transkriptomovou analýzu

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Title in English Using of magnetic micro-beads for analysis of transcriptome
Authors

HUŠKA Dalibor PETRLOVÁ Jitka ADAM Vojtěch TRNKOVÁ Libuše HAVEL Ladislav KIZEK René

Year of publication 2006
Type Article in Proceedings
Conference Vyziva zvirat 2006 - PROTEINY
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords DNA; RNA; hybridization; electrochemical detection; adenine
Description A simple, rapid and low cost detection of DNA and RNA sequence is a difficult task for many scientists around the world. Here, we suggest utilizing paramagnetic micro-beads in connection with electrochemical detection of adenine to study of transcriptome (total mRNA). Moreover time of hybridization of nucleic acid on the surface of the beads has been investigated. Recovery of the analysis is sufficient.
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