Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Medical Microbiology |
| MU Faculty or unit | |
| Citation | |
| web | [Abstract, Full text] |
| Field | Microbiology, virology |
| Keywords | Staphylococcus equorum; Staphylococcus succinus; identification; ribotyping; human clinical material |
| Description | A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/ Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII and wholle-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a previously described PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S.xylosus, S. equorum or S. succinus. The present study confirms what is believed to be first occurrence of S. equorum strains in the relevant human clinical material in the Czech Republic and describes what is believed to be first-ever isolation of S. succinus from human clinical material. |
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