Molekulární typizace bakterií rodu Lactobacillus pomocí rep-PCR

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Title in English Molecular typization of bacteria of Lactobacillus genera using rep-PCR
Authors

DUŠKOVÁ Marta ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2006
Type Article in Proceedings
Conference X. Pracovní setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Typization; rep-PCR ; Lactobacillus
Description The aim of the work was the use of rep-PCR for molecular typisation of lactic acid bacteria of genus Lactobacillus. Alltogether 38 collection strains and 20 new isolated strains (of human origin) were used in experiments. DNA was isolated using phenol extraction method. Concentration and purity of DNA was estimated spectrophotometrically. The quality of purified DNA was proved using agarose gel electrophoresis and using PCR with genus specific primers LbLMA 1-rev and R16-1 (Duberbet et al., 2002). Typisation of bacteria of genus Lactobacillus was carried out using rep-PCR primer (GTG)5 (Gevers et al. 2001). Tested bacterial strains of genus Lactobacillus can be divided into groups which reflect their intraspecies heterogenity.
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