Amesův test jako metoda detekce rizikových látek - praktické cvičení pro studenty.
| Title in English | Ames` test as a method of danger substances detection - practical exercise for students. |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Sborník z celostátní konference s mezinárodní úastí: XXXIII. Ostravské dny dětí a dorostu. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Ames` test-mutagenity-genotoxicity- student-training. |
| Description | Ames' test - synonym: Ames assay. A widely used screening test for the potentially carcinogenic effect of substances their mutagenic effect on microorganisms, cells- and tissue cultures. It relies on the observation that the most common cause of cancer is somatic mutations brought about by DNA damage. Chemicals that damage bacterial DNA, and induce mutations, are also likely to cause mutations in mammalian cells. Doing the assay in bacteria gives much faster and less expensive results than animal studies, with results usually being available in 2 days. With this method it is possible to assay directly the mutagenic and teratogenic effect of the substances in tobacco smoke. Ames used mutants of Salmonella enterica (formerly S. typhimurium). Ames test was modified for use in hands teacher training. |
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