Amperometric flow-injection determination of phenolic compounds using a biosensor with immobilised laccase, peroxidase and tyrosinase

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Authors

SOLNÁ Renáta SKLÁDAL Petr

Year of publication 2005
Type Article in Periodical
Magazine / Source Electroanalysis
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords electrochemical biosensor; enzyme electrode; phenol; catechol; waste water
Description A screen-printed four-electrode sensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in the same array was developed for monitoring of phenols. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol) modified gold surface via covalent attachment by epichlorohydrin coupling. The experimental conditions for simultaneous operation of the three enzymes were optimized based on catechol determination. The sensors were further applied for the amperometric detection of several substituted phenolic compounds, carried out using a single line flow-injection system. Hydrogen peroxide served as co-substrate for peroxidase. The limits of detection for phenols in aqueous solutions were in the micromolar range, one assay was completed in less than 5 min. The preliminary studies showed that the compatibility of the above mentioned enzyme array enabled the multielectrode biosensor to be applied to real samples including industrial wastewaters and surface waters.
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