Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Folia Microbiologica |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Lactococcus lactis; PCR; rep-PCR; RAPD |
| Description | A collection of 34 lactococcal strains were characterized using PCR for acmA gene, and for 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindromes-PCR (rep-PCR). PCR experiments enabled the genotypic identification of L. lactis strains by RAPD. Rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterizations. |
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