Purification and some new kinetic parameters of FerB enzyme from Paracoccus denitrificans

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Authors

SEDLÁČEK Vojtěch KUČERA Igor JANICZEK Oldřich

Year of publication 2005
Type Article in Proceedings
Conference ChemZi
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords ferric quinone reductase radicals Cibacron dicumarol
Description A Cibacron Blue Agarose chromatography was introduced as the first step in FerB purification from cell-free extracts. Kinetic parameters (KM, Vlim) were determined for a series of benzoquinones and naphtoquinones differing in their lipophilicity and redoxpotential. FerB was also characterised with respect to reaction mechanism and senstitivity to the known inhibitors of quinone reductases.
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