Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis

Investor logo

Warning

This publication doesn't include Faculty of Sports Studies. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

KŘÍŽOVÁ Jana ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2004
Type Article in Proceedings
Conference The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Bifidobacterium spp.; identification; genus and species specific PCR; ARDRA
Description Molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info